The list contains links to test guidelines, method protocols, computational tools and databases, and guidances and policies from EPA  that "do not use vertebrate animals to develop new data/information," and additional guidance or other documents that are considered "tools and approaches which may enhance the use of NAMs for regulatory use under TSCA."

New additions to the list include:

- The ToxCast Estrogen Receptor Agonist Pathway Model
- In vitro BALB/c 3T3 Cell Transformation Assay
- Determination of in vitro intrinsic clearance using rainbow trout hepatocytes (OECD TG NO. 319A)
- Determination of in vitro intrinsic clearance using rainbow trout liver S9 sub-cellular fraction (RT-S9) (OECD TG NO. 319B)
- Several guidance documents related to waiving or bridging or alternative approaches for acute systemic toxicity, eye irritation, skin sensitization, and endocrine disruption
- FIFRA SAP reports on endocrine disruption, respiratory toxicity, and the EPA CompTox Chemicals Dashboard

More information here:
https://www.epa.gov/assessing-and-managing-chemicals-under-tsca/strategic-plan-reduce-use-vertebrate-animals-chemical