With the new imaging technique, it was possible to transilluminate complete cells and map their structure in three dimensions within five to ten minutes. In addition, the researchers led by Prof. Dr. Ralf Bartenschlager from the Department of Molecular Virology at Heidelberg University Hospital and Dr. Venera Weinhardt from the Centre for Organismal Studies (COS) at Heidelberg University were able to detect accumulations of SARS-CoV-2 particles on cell surfaces and identify virus-related changes in the cell interior. This allowed them to visualize structures that may serve viral replication and spread.
Original publication:
V. Loconte, J.-H. Chen, M. Cortese, A. Ekman, M. A. Le Gros, C. Larabell, R. Bartenschlager, V. Weinhardt; "Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells"; Cell Reports Methods; Vol. 1, Issue 7, 22 November 2021, 100117.
Source and further information:
https://www.uni-heidelberg.de/de/newsroom/in-nur-wenigen-minuten-zellstrukturen-dreidimensional-abbilden