To immunize, researchers at the Institute of Biology and Technologies in Sacley (CEA IBITECS) use a fusion protein composed of three components: an antigen (from an infectious organism or a cancer cell) and two proteins that trigger the immune response.
The immune response is initiated by the culture of blood cells of a healthy donor with the fusion protein in a test tube. The fusion protein activates B-cells which produce the desired antibodies (specifically acting immunoglobulin G).
In a next step, the researchers want to isolate individual B cells to produce antibodies in larger quantities.
The researchers have submitted their technique for a patent.
Source:
https://idw-online.de/en/news629840
Press release of the CEA:
http://portail.cea.fr/actualites-scientifiques/pages/sante-sciences-du-vivant/therapies-ciblees--un-brevet-pour-fabriquer-in-vitro-des-anticorps-pleinement-humains--.aspx
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Monday, 27 April 2015 13:14
France: In vitro production of human antibodies Featured
Most of the strategies for the preparation of antibodies are still based on the immunization of humanized mouse models. To replace this procedure, there are several methods, for instance, the phage display. French researchers now have turned to another method: they improved the ability of immunization of blood cells in vitro.
Dr. rer. nat.
Menschen für Tierrechte - Tierversuchsgegner Rheinland-Pfalz e.V.